Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------MYCPQCGHQTDGGNFCEKCGSPLPGQSGHQHAAQTGA-AAKQA-AKQFGSFVLSVLKRPYQECKATGGEQ------------LISAIITMVLFS----------LLTPLMF-YILFSDGPGSVSFTAIF-L----------------------------EPTIYFILFLFGLHACIFFALKIAGNQVSFKDSFSRFGAFLIPFTAILILALFF--FLLHTDICFTILAVGLIGAFFAI----PPAMLSSYQHSYKGKV---DFIYSTIVIYLII------CVTFQLIIEHYVKEIFRYMLF----------- 2BI0 Chain:A ((8-337)) IRVGGPYFDDLSKGQVFDWAPGVTLSLGLAAAHQSIVGNRLRLALDSDLCAAVTGMPGPLAHPGLVCDVAIGQSTLATQRVKANLFYRGLRFHRFPAVGDTLYTRTEVVGLRANSPKPGRAPTGLAGLRMTTIDRTDRLVLDFYRCAMLPASPDWKPGAVPGDDLSRIGADAPAPAADPTAHWDGAVFRKRVPGPHFDAGIAGAVLHSTADLVSGAPELARLTLNIAATHHDWRVSGRRLVYGGHTIGLALAQATRLLPNLATVLDWESCDHTAPVHEGDTLYSELHIESAQAHADGGVLGLRSLVYAVSDSASEPDRQVLDWRFSALQF

General information: TITO was launched using: RESULT: Template: 2BI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1055 -77785 -73.73 -335.28 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -73.73 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.073

(partial model without unconserved sides chains): PDB file : Tito_2BI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BI0-query.scw PDB file : Tito_Scwrl_2BI0.pdb: