Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENMSFKELYAIVRHRFVLILLITIGVTLIMGFVQFKVISPTYQASTQVLVHESDGEENSNLSDIQRNLQYSSTFQSIMKSTALMEEVKAELHLSESASSLKGKVVTSSENESEIINVAVQDHDPAKAAEIANTLVNKFEKEVDERMNVQGVHILSEAKASESPMIKPARLRNMVMAFGAAVMGGITLAFFLHFLDDTCKSARQLSERTGLPCLGSVPDVHKGRNRGIKHFGE 1YZ7 Chain:A ((214-254)) ---------------------------------------------------------------------------------------------------------VKFTYL-GAPRYRIDITAPDYYKAEEVLESIAEEILRVIKEA---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1YZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 55 -15894 -288.98 -387.66 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -288.98 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.758

(partial model without unconserved sides chains): PDB file : Tito_1YZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YZ7-query.scw PDB file : Tito_Scwrl_1YZ7.pdb: