Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MK----TASKFSV----MFFMLLALCGCWDVKDIEQLSFARGLAIDETN-DHQYKLTYQNLLPQSEDSQASGKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKIMIPLRIGRASVYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVGFLSADETQTLSWVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTEDQ-NPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 1D9E Chain:A ((1-284)) MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTVTQKLGIPYVFKASFDKANRSSIHSYRGPG------LEEGMKIFQELKQTF--GVKIITD-VHEPSQAQPVADVVDVIQLPAFL-----ARQTDLVEAMAKTGAVINVK-KPQFVSPGQMGNIVDKFKEGG-NEKVILCDR-------------------------------GANFGYDNLVVDMLGFSIMKKVS---------------GNSPVIFDVTHALQR-AQVAELARAGMAVGLA--GLFIEAHPDPEPSALP---LAKLEPFLKQMKAIDDLVKGFEELDTSK--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1D9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1471 65121 44.27 254.38 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 44.27 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_1D9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D9E-query.scw PDB file : Tito_Scwrl_1D9E.pdb: