Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAVSVILTLSLFLLTACSLEPPQWAKPSNSGNKKEFTIGLSVSTLNNPFFVSLKKGIEKEAKKRGMKVIIVDAQNDSSKQTSDVEDLIQQGVDALLINPTDSSAISTAVESANAVGVPVVTIDRSAEQGKVETLVASDNVKGGEMAAAFIADKLGK-GAKVAELEGVPGASATRERGSGFHNIA--DQKLQVVTKQSADFDRTKGLTVMENLLQGHPDIQAVFAHNDEMALGALEAINSSGK-DILVIGFDGNKDALASIKDRK-LSATVAQQPELIGKLATEAADDILHGKKVQKTISAPLKLETQK 4YV7 Chain:A ((39-312)) -----------------------------------GALKIGFSQATQQSPFYVALTDAAKAEAQAQGDELFYADANGDITKQNNDVQDLITRGINVLVINPVDPKGVTPSLAAAEAAGIKVVTVDRPV-ESGAASFVGRDNKAMGELVGKAAVDTLGPDGGKIIEIQGDAGGAVARDRRDGFQAAVSGRPNITIVEGPYCDYIRSKAVTAMQDLLQAHPDLKGVYAQNDDMALGAMQVLAENNRTDVKVFGVDGLMEAVRAIADGDQYVATALNDPDAEGRLAIQTAAKVARGESVPEFVDAGTGLVDKS

General information: TITO was launched using: RESULT: Template: 4YV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1628 21141 12.99 78.59 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 12.99 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_4YV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YV7-query.scw PDB file : Tito_Scwrl_4YV7.pdb: