Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDIHCHILPAMDDGAGDSADSIEMARAAVRQGIRTIIATPHHNNGVYKNEPAAVREAADQLNKRLIKEDIPLHVLPGQEIRIYGEVEQDLAKRQLLSLND------------------------------------------------------TKY--ILIEF------------------PFD--HVPRYAEQLFYDLQLKGYIPVIAHPERNREIR--ENPSLLYHLVEKGA-ASQITSGSLAGIFGKQLKAFSLRLVEANLIHFVASDAHNVKTRNFHTQEALYVLEKEFGSELPYMLTENAELLLRNQTIFRQPPQPVKRRKLFGFF 3O0F Chain:A ((14-267)) GWDIHCHTVFS--DGTETPRT---LVEQARKLGLHGVAIADHDTTAGWD-----------EATEASE--EIGLPLLLGTEITAVDEDVS----VHMLAFQYDPSNEHISSMFANTRAARLRRTKRMVERLSQDFPITWDDVLAQVKEGERTTIGRPHIADALVAAGVYETRSDAFADAVSAKSKYYIPTPSPSTHEVIAAVKGAGGVVVAAHAGDPQRNRRLLSDEQLDAMIADGLDGLEVWHRGNPPE---QRERLLTIAARHDLLVTGGSDWHGKGK------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3O0F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 847 -27195 -32.11 -155.40 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -32.11 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_3O0F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O0F-query.scw PDB file : Tito_Scwrl_3O0F.pdb: