Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFKLVDLNVERVDKEEQSIEQFPLIDGLIINKEDGENHWLIEALVQKEHLSFFEQLQNSQSEAKVFVTITKKSNRPAQLTAAVKNIVKLEESIQVLLYGQMVTRKQQGTETILESLVKEGYTGTKLIEAFKQKL- 1CY5 Chain:A ((1-92)) MD-----AKARNCLLQHREALEKDI------------KTSYIMDHMISDGFLTISEEEKVRN-----------EPTQQQRAAMLIKMILK---------------KDNDSYVSFYNALLHEGY--KDLAALLHDGIP

General information: TITO was launched using: RESULT: Template: 1CY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 358 -27647 -77.23 -303.81 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -77.23 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_1CY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CY5-query.scw PDB file : Tito_Scwrl_1CY5.pdb: