Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREEEKKTSQVKKLQQFFRKRWVFPAIYLVSAAVILTAVLWYQSVSNDEVKDQLADNGGNSAYDNNDDAVEVGKSMENVAMPVVDSENVSVVKKFYETDAAKEEKEAALVTYNNTYSLSKGIDLAEKDGKDFDVSASLSGTVVKAEKDPVLGYVVEVEHADGLSTVYQSLSEVSVEQGDKVKQNQVIGKSGKNLYSEDSGNHVHFEIRKDGVAMNPLNFMDKPVSSIEKAATQETEESIQQSSEKKDGSTEKGTEEKSGEKKDDSTDKSGSKESSTTEDTEQS 3SLU Chain:A ((219-345)) ------------------------------------------------------------------------------NIEPLVY---TRISSPFGYRMHPI----------LHTWRLHTGIDYAAPQGT--PVRASADGVITFKGRKGGYGNAVMIRHANGVETLYAHLSAFSQAQ-GNVRGGEVIGFVGSTGR--STGPHLHYEARINGQPVNPVSVALPT------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3SLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 650 -30522 -46.96 -240.33 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -46.96 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_3SLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SLU-query.scw PDB file : Tito_Scwrl_3SLU.pdb: