Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYA----ISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCG-SPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 4UXJ Chain:F ((12-182)) -------GRIELIIGPMFAGKTTELMRRVKREIHARRSCFVIKYSKDTRYD------HDQLMLRAQAAVSQLTEVRDTWKRF----DVLAIDEGQFF-SDLVDFCNTAADAGKVVMVSALDGDYRRKPFGQICELVPYCEAVDKLTAVCMMCHEQPACFTRRTVNVEQQE------LIGGADMYIATCR-----------

General information: TITO was launched using: RESULT: Template: 4UXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 780 -17876 -22.92 -111.72 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain F : 0.77 3D Compatibility (PKB) : -22.92 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_4UXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UXJ-query.scw PDB file : Tito_Scwrl_4UXJ.pdb: