Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKVSVIMTSYNKSDYVAKSISSILSQTFSDFELFIMDDNSNEE------TLNVIRPFL-N-DNRVRFYQS----------------------D-ISGVKERTEKTRYAALINQAIEMA---EGEYITYATDDNIYMPDRLL----KMV-RELDTHPEKAVIYSASKTYHLNENRDIVKETVRPAA-QVTWN--AP-----CAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHF-YPFYPLDEELDLNYITDQSIHFQLFELEKNEFVRNLPPQRNCRELRESLKKLGMG 2ZU9 Chain:A ((51-284)) -GKMAVIVPMKNEKL---HLVDGVLKAIPHKCPIIIVSNSKREGPNRYKLEVDLIRHFYNLTHSKIIMIHQKDPGLAKAFKEVGYTDILDENGMIRSG-------K-GEG-MLVGLLLAKAIGAEYVGFVDAD-NYIPGAVNEYVKDYAAGFLMSESEYTMVRLHW--R--------VSEITNHYLNLLVSEHTAFETTIMVTGNAGEHAMTMKLAEIL-----PFS---TGY--SIEPYEIVYILERFGK-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 648 -20111 -31.03 -119.00 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.03 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_2ZU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZU9-query.scw PDB file : Tito_Scwrl_2ZU9.pdb: