Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRTEKEKMAAGELYNSE-DQQLLLERKHARQLIRQYNET-PEDDAVRTKLLKELLGSVGDQVTILPTFRCDYGYHIHIGDHTFVNFDCVILDVCEVRIGCHCLIAPGVHIYTAGHPLDPIERKSGKEFGKPVTIGDQVWIGGRAVINPGVTIGDNAVIASGSVVTKDVPANTVVGGNPARILKQL 3V4E Chain:B ((5-187)) ---TEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATNKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKI

General information: TITO was launched using: RESULT: Template: 3V4E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 951 -16177 -17.01 -89.38 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -17.01 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_3V4E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V4E-query.scw PDB file : Tito_Scwrl_3V4E.pdb: