Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKVHQSACPLNCWDSCGFLVTVDDGKVTKVDGDPNHPITEGKICGRGRMLETKTNSPDRLRYPMKKQN---------------GEFVRISWEQALDEIADKLREIKETSETTAVLHSHDY-ANNGLLKALDQRFFNGYGGV--TEIV--GSICWGSGIEAQSWDFGRSY------G--HGPLDIYNSKHVVVWGRNVSRTNMHLY--------HHL---QQV-KKKGATITVIDPIFNPTAK-LADRYISVKPGMDGWLAAAVLKVLIEMGRTDETFISEHSVGFDDVKELLK------TVSLEEFIVKTETSMEELEYLAGLYADGPVSTFMGLGMQRYKNGGGTIRWIDALVAASGNVGIKGGGANFGNVQI-GESFA--KTKL-TLPEL-------KT-------TSRSFSMMTQAEEVL--------T-A-ADPA-IEMIIVTCGNPLTQVPNTNKVRQAFEKVPMTVAIDSIMTDTAELCDYVLPTATVFEEEDIYYS---SMYHHYVQYGKKLVEPQGEAKSDSWIWSELAKRLGFGELFE--YSTQEFLEMGLSSL-----E-AEDVTLERLKEKGHLPLP-------VKQV--PWDDYQFLTPSGKFEFTSSLAEQKGFSGSLQLNVPEESV-FHNEE------LAGKYPYTLLSIHPQRSNHSQ--HVPFIEKL----QHVQVDISPDIAAGQDLQDGDEVVIFNDRGSMKGKVKVMKQAHAKTINIDEGMWAAF------------------GGSVNALTNDTNSDN-GMGSTLFDCLVGLKKA 1TMO Chain:A ((39-811)) ------EWLT-TGSHFGAFKMKRKNGVIAEVKPFDLDKYP----TDMINGIRGMVYNPSRVRYPMVRLDFLLKGHKSNTHQRGDFRFVRVTWDKALTLFKHSLDEVQTQYGPSGLHAGQTGWRATGQLHSSTSHMQRAVG-MHGNYVKKIGDYSTGAGQTILPYVLG--STEVYAQGTSWPLI-LEHSDTIVLWSNDPYKNLQVGWNAETHESFAYLAQLKEKVKQGKIRVISIDPVVTKTQAYLGCEQLYVNPQTDVTLMLAIAHEMISKKLYDDKFIQGYSLGFEEFVPYVMGTKDGVAKTPEWAAPICGVEAHVIRDLAKTLVKGRTQFMMGWCIQRQQHGEQPYWMAAVLATMIGQIGLPGGGISYGHHYSSIGVPSSGAAAPGAFPRNLDENQKPLFDSSDFKGASSTIPVARWIDAILEPGKTIDANGSKVVYPDIKMMIFSGNNPWNHHQDRNRMKQAFHKLECVVTVDVNWTATCRFSDIVLPACTTYERNDIDVYGAYAN--RGILAMQKMVEPLFDSLSDFEIFTRFAAVLGKEKEYTRNMGEMEWLETLYNECKAANAGKFEMPDFATFWKQGYVHFGDGEVWTRHADFRNDPEINPLGTPSGLIEIFSRKIDQFGY-----DDCKGHPTWMEKTERSHGGPGSDKHPIWLQSCHPDKRLHSQMCESREYRETYAVNGREPVYISPVDAKARGIKDGDIVRVFNDRGQLLAGAVVSDNFPKGIVRIHEGAWYGPVGKDGSTEGGAEVGALCSYGDPNTLTLDIGTSKLAQACSAYTCLVEFEKY

General information: TITO was launched using: RESULT: Template: 1TMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3400 53661 15.78 83.20 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 15.78 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1TMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TMO-query.scw PDB file : Tito_Scwrl_1TMO.pdb: