Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MSIQHRTQDAIRTLTAAFAPFD-CLIQATRKGNFSFTIVDQHGVACHSERLYPEQYDDSGKMEEVINRVRKKTLAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4U5B Chain:A ((1-804)) NSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKGERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGAEVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAG---------DCLANPAVP--WGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTEEQKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWDGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMAIVFAYILVSVVLFLSARIVAGVWWFFTLIIISSYTANLATFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGASALSLSNVAGVFYILVGGLGLAMLVALIEF

General information: TITO was launched using: RESULT: Template: 4U5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -7406 for 318 contacts (-23.3/contact) + 2D Compatibility (PS) -6512 + (NN) 1961 + (LL) 912 1D Compatibility (HY) -2000 + (ID) 750 Total energy: -13795.0 ( -43.38 by residue) QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_4U5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U5B-query.scw PDB file : Tito_Scwrl_4U5B.pdb: