Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKYMSLLTRIEEMMQSTSYQEGDRLPSIRQLSARYQVSKSTVIRALQELEKRHLIYSVPKSGYYIVKKSGKSKS-----------GQPGPIDFATSAPDPDVFPYLDFQHCINKAIDTYKNDLFIYGTPKGLPSLIRVLRKLLAT-QQVFADERHIFITSGVQQALSLLCAMPFPNGKEKIAIEQPGYHLMVEQLETLGIPAIGVKRTEEGLDIAKVERLFQTESIKFFYTMPRFHNPLGCSLSERDKQELVRLAEAY-DVYLVEDDYLGDLEENKK-ADPLYAYDLSSHVIYLKSFSKMMFPGLRVGAAVLPEALTDTFYAYKKLNDIDCSMISQAALEIYLKSGMYGRHKEKIRDSYKERSLRLHQAIRTHRQLGSGRFTFSSGQAPCMHTHLVLPQDLPASRVIHRLEKQGVLLEAIDRHYLSDYPKENLLKINISNVKTEDIERGVKLLMSHL 4TV7 Chain:A ((15-463)) -YIYQQIYQKLKKEILSRNLLPHSKVPSKRELAENLKVSVNSVNSAYQQLLAEGYLYAIERKGFFVEELDMFSA-ALPDDLKEIHI----WISFSHM--DTDHFPIKSWFRCEQKAASRSY-----MSHPQGIYEVRAAITRLISLTRGVKCRPEQMIIGAGTQVLMQLLTE-LL-PKEAVYAMEEPGYRRMYQLLKNAGKQVKTIMLDEKGMSIAEITR----QQPDVLVTTPSHQFPSGTIMPVSRRIQLLNWAAEEPRRYIIEDDYDSEFTYDVDSIPALQSLDRFQNVIYMGTFSKSLLPGLRISYMVLPPELLRAYKQR-GYDLQTCSSLTQLTLQEFIESGEYQKHIKKMKQHYKEKRERLITALEA--EFSGE-VTVKG-ANAGLHFVTE-DTRRTEQDILSHAAGLQLEIFGMSRF-------RPALIIGFARLKEEDIQEGVQR-----

General information: TITO was launched using: RESULT: Template: 4TV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1979 -228621 -115.52 -561.72 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -115.52 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_4TV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TV7-query.scw PDB file : Tito_Scwrl_4TV7.pdb: