Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRIILLLAVIIAAAAAGVAF-YVAKDKGHEKAADVSVNTESGDELLVSITDTDLLTKYYENDKVIHEEKLTSYPAFALDQKQQVLYYT--------GNNEQN-EMRL-----FKLDLKSHKKTMLYKGAESADSLFLSKDRSTIYFRLGKADE-SNFRIAAFDLKTKKYKNLYPAANDQDDTVSSFFYNQKNDSFALLHYSV--------EEDYKKTDEANEKGIDPEPTTIHFAEGRQNKFNELKSLNQF-------ISDIAVSDDDKRILFT----SYTQKGTEQTASIQMLNADTKKYESIISNQKSFKLLIDAQPQFSKDGKNIYFLAEAKG-AKKLKDE-----------------TGREA-KVRTIYSYSLENKTFKK-VWENPNGIINSFF---VIN 3WJ9 Chain:A ((3-412)) QKSQFAYRSSKSIGLVNASENYASPPKFEAISEPARNACYSPNG-KLFAYATATQVVINDTESGAKLTQLPAANTYELGFSPLGKYLSTWERPGKEADGTPKQNMKVWNTETGQLVFSFVQRNQTGWNLQYTCDESLAARLVTNEVHFYET--GNMSKGPIAKLRVEGISDFALSPG---QNHAVAVFIPEKKGAPASVRTYSIPNFNSPLSQKTFFKADKVQFKWNALGTSLLVLTQDKSNKNYYGETTGQFDLDREGPIHDVCWNADSKEFGIVYGYMPAKTAIFDNRANVVSIIPPAPRNTLIFSPNSRYILLAG----FGNLQGSIDIFDAANNMKKITTVEAANCTYCEFSPDSQFLLTAVTSPRLRVDNSIKIWHITGAPMFYEEFNELYQAFWRPRPLN

General information: TITO was launched using: RESULT: Template: 3WJ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1720 -65824 -38.27 -194.74 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -38.27 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.271

(partial model without unconserved sides chains): PDB file : Tito_3WJ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WJ9-query.scw PDB file : Tito_Scwrl_3WJ9.pdb: