Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQTILSNGIAMVLIILIINIVYVSFFTIRMILTLKGQRYLAAGISTIEILVYVTGL----SLVLDNLD--QIQNVIAYALGYGLGVIVGMKIEEKLALG---YIMVNVITKELDLDLPKQL-REKGYGVTNWVAGGLEGDRTA-----LQIL---TPRRYELQLYD------TIKTLDSKAFIIAYEPKTIHGG---FWVKAVKKRRIKE 1QHL Chain:A ((4-224)) ------RGKFRSLTLINWNGFFARTFDLDELVTTLSGGN--GAGKSTTMAAFVTALIPDLTLLLHGKLKAGVCYSMLDTINSRHQRVVVGVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAMEGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLRDYLLPE

General information: TITO was launched using: RESULT: Template: 1QHL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 800 -111631 -139.54 -634.27 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -139.54 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.136

(partial model without unconserved sides chains): PDB file : Tito_1QHL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QHL-query.scw PDB file : Tito_Scwrl_1QHL.pdb: