Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLLRFDVIEGRDEKSLQLLLDTAHQAMVEAFGVPERDRYQIVHQHPANELIIQDAGLGFQRTKDMVIISMTSKA-RTESQKEKLYALLAERLEKKCEISPDDLMVSITENGDADWSFGLGEAQFLNGKL 4LHO Chain:D ((1-128)) -PLIRIDLTSDRSREQRRAIADAVHDALVEVLAIPARDRFQILTAHDPSDIIAEDAGLGFQRSPSVVIIHVFTQAGRTIETKQRVFAAITESL-APIGVAGSDVFIAITENAPHDWSFGFGSAQYVTGEL

General information: TITO was launched using: RESULT: Template: 4LHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 501 -50968 -101.73 -401.32 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain D : 0.85 3D Compatibility (PKB) : -101.73 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_4LHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LHO-query.scw PDB file : Tito_Scwrl_4LHO.pdb: