Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPTKQHKKETFTHF-IFRVF---------------------MILVGAAS--AAVSIELFLI-------PNDFIDGG----------IIGVSLILDHLFMSNPFLNFAFFVVILNIPFMIFGYKYIGKTFLVSTFIGIVGLAVIESSLHHVEAITTQPILATVFG-GLLLGFGVGLVIRNGGSMDGTE-ILGILLTKK-LPFSVGE----FVMFI---NVFIFIWAVFVFGPEQAMYSFMTYYIAMKTIDAVIQGLDETKA----VIIV-----SEQYDEISDAILHRLGRGTTKLKGKGGYTD-EEKDVIYAV----------VTRLEVTKLKSIVFEVDQNAFITIMNTHETR--GGKFKNAIH-------------------- 3FHC Chain:A ((9-405)) -IPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAQKRPFAYHKLLKDAGGMVIDMKWNP----TVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNAPVLMLLSTDGVLCPFYMIN

General information: TITO was launched using: RESULT: Template: 3FHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1396 -120915 -86.62 -419.84 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -86.62 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_3FHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHC-query.scw PDB file : Tito_Scwrl_3FHC.pdb: