Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAID---ENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR-GPHKFDVKR---F-NASFAYTVDGGPL----GELEYESFNAAAAKITIKG--NNVHPGTAKGKMINSAKIAMKLNSLLPADEAPE--------------------------------------------------YTEGYEGFYHLLSIQGD----------VEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMEN-LGIE--PKISPIRGGTDGSQLSYMGLPTPNIFTGG--ENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 2POK Chain:A ((44-481)) --QHYFEVLRTLISKKSVFAQQ-------VGLKEVANYLGEIFKRVGAE-VEIDESYTAPFVMAHFKSS-RPDAKTLIFYNHYDTVPADGDQVWT---EDPFTL-SVR----------------------NGFMYGRG----VDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYG-GVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGF--D--KVELYYTLGEMSYRS--DMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE---

General information: TITO was launched using: RESULT: Template: 2POK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1582 5422 3.43 15.10 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 3.43 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_2POK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2POK-query.scw PDB file : Tito_Scwrl_2POK.pdb: