Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIDLQKKSVKIVLEKKQLTKVTARVGLVLDITGSMRPL--YKNGTVQNVVERILAVADQF-DDNGLLDVWVYDNEFSRLKPVSEKDFSGYVDREILNNDRLHKFGRNDEPPVMKDVLRKYVTEEPSSYPAFIVFINDGGCKK--SIKPIIEASSDKPVFWQFVGIGN-GNFDFLNKLDTLEGRVIDNTNFLHIEEIDRISDDELYDALLAEFPFWLKEAKEKGIVREQEPPAEKPKKKGFFSRLFSK 4CN9 Chain:A ((251-440)) ----------------------GHADIAFVFDASSSINANNPNNYQLMKNFMKDIVDRFNKTGPDGTQFAVVTFADRATKQFGLKDYSSKADIKGAIDKV-TPSIIGQTAIGDGLENARLEVFP--REEVQKVVILLTDGQNNGHKSPEHESSLLRKEGVVIVAIGVGTGFLKSELINIASS------EEYVFTTSSFDKLSK--IMEDVVKLACMS--------------------------------

General information: TITO was launched using: RESULT: Template: 4CN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1020 -118188 -115.87 -663.97 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -115.87 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_4CN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CN9-query.scw PDB file : Tito_Scwrl_4CN9.pdb: