Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSA-Q---PKADTQAYIQNWADQYSQYDRFLKGE---------NAYVAGVLYDFLEKARASNVSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAEAREKAEIKLETSL--RSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKNGAHEGMGTYIIQKLVKGAFGRLDFTYRHPIFRLEIKIPFQK 4QPK Chain:A ((45-242)) --------------------------------------------------------------------------------------------------------------------------DLGALLCSRICHDIISPIGAINNGLELLEEGGADEDAMALIKSSARNASARLQFARIAFGAAGVQIDTGDAQNVATEYFRNEKPEFTWEGAR---V--LLPKNKVKLLLNMLLIGNGAIPRG---GSLAVRLEGSDTDPRFVITVKGRMLRVPPKFLELHSG-AAPEEPI-DAHSVQPYYTLLLAEEAGMKISIHATAEDIVFSA------

General information: TITO was launched using: RESULT: Template: 4QPK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 760 -69592 -91.57 -386.62 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -91.57 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.337

(partial model without unconserved sides chains): PDB file : Tito_4QPK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QPK-query.scw PDB file : Tito_Scwrl_4QPK.pdb: