Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDMPVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDELYKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGF--EWGGDWKR-----------FKDYPHLEMIPN 2MXZ Chain:A ((5-137)) -------------------------------FGKNSEKQLATVKPELQKVARRALE---LSPYDFTIVQGIRTVAQSAQNIANGTSFL------KDPSKSKHITGDAIDFAPYIN-GKIDWNDLEAF--WAVKK--AFEQAGKELGIKLRFGADWNASGDYHDEIKRGTYDGGHVELV--

General information: TITO was launched using: RESULT: Template: 2MXZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 -23830 -51.36 -198.58 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -51.36 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.192

(partial model without unconserved sides chains): PDB file : Tito_2MXZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MXZ-query.scw PDB file : Tito_Scwrl_2MXZ.pdb: