Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTKGLASTAPEIMIARIAAQTNTIRVGSGGVLLPQYSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTDG---VKKSLTEFNRQLQDVSYFLTDSLP-PDHPYAGIK------------AAPLI--GTAPELWVLGLG--ENSARRAAHQGIGYVFGHFINPER-GENAFRIYRESFRPSAHF-SNPSALFTIFVICAKTDEEAEELALSQ-DLWLLRVGKG-LD--SRVPSIEEAKAHPYTASDKKLIEENR-KRMVIGSPTTVKQQLLDLTGCY-ETNEIMVLCNV--FDFEAKKESYERLAELFL 4UWM Chain:A ((2-370)) AMETGLIFHPYMRPGRSARQTFDWGIKSAVQADSVGIDSMMISEHASQIWENIPNPELLIAAAALQTKNIKFAPMAHLLPHQHPAKLATMIGWLSQILEGRYFLGIGAGAYP--QASYMHGIRNAGTKNLNDMVRESLFIMEKIWKREPFFHEGKYWDAGYPEELEDEQHKLADFSPWGGKAPEIAVTGFSYNSPSMRLAGERNFKPVSIF-SGLDALK-RHWEVYSEAAIEAGHTPDRSRHAVSHTVFCADTDKEAKRLVMEGPIGYCFERYLIPIWRRFGMMDGYAKDAG-ID-PVDADLEFLVDNVFLVGSPDTVTEKINALFEATGGWGTLQVEAHDYYDDPAPWFQSLELISKEVA

General information: TITO was launched using: RESULT: Template: 4UWM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1734 -163878 -94.51 -504.24 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -94.51 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_4UWM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UWM-query.scw PDB file : Tito_Scwrl_4UWM.pdb: