Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MQDLYQLIGEKLNDIIPGEWTKI-YLYAEVLDDSTMVLFHFRTPENNQIIYSQDIPSHYNVSKDIFKTLLREL-RELFEELRTEHRNNNDDVWTNLTLTLDRSGEFQLDYNYDDILASELDGYERIAIWEYKN-----LGILPEDEDDKEFVISYLGL------------------------------------------------------------------------------- 4JC0 Chain:A ((2-433)) RVGIKVLGCPKNEADCEVLAGVLREGGHEIVFDVKDADVVVLDTCAFIEDAKRESIDEIFSFVDAKDQYGYKLVVKGCLVQRYYEELKKEIPEVDQWIGVADPEEIANAIETDLVPDRYRKRIDLEERPYAYVKISDGCDRGCTFCSIPSFKGSLRSRSIEDITREVEDLLKEGKKEIILVAQDTTSYGIDLYRKQALPDLL-RRLNSLNGEFWIRVMYLHPDHLTEEIISAMLE----LDKVVKYFDVPVQH-GSDKILKLMGRTKSSEELKKMLSSIRERFPDAVLRTSIIVGFPGETEEDFEELKQFVEEI-QFDKLGAFVYSDEEGTVAFNLKEKVDPEMAKRRQEELLLLQAEISNSRLDRFVGKKLKFLVEGKEGKFLVGRTWTEAPEVDGVVFVRGKGKIGDFLEVVIKEHDEYDMWGSVILEH

General information: TITO was launched using: RESULT: Template: 4JC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 594 -50414 -84.87 -350.10 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -84.87 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_4JC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JC0-query.scw PDB file : Tito_Scwrl_4JC0.pdb: