Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQSFNAPYEAIG-EELLSQLVDTFYERVASHPLLKPIFP-SDLTETARKQKQFLTQYLGGPPLYTEEHGHPMLRARHLPFPITNERADAWLSCMKDA---MDHVGLEGEIREFLFGRLELTARHMVNQTEAEDRSS 1NGK Chain:B ((2-125)) -PKSF---YDAVGGAKTFDAIVSRFYAQVAEDEVLRRVYPEDDLAGAEERLRMFLEQYWGGPRTYSEQRGHPRLRMRHAPFRISLIERDAWLRCMHTAVASIDSETLDDEHRRELLDYLEMAAHSLVN---------

General information: TITO was launched using: RESULT: Template: 1NGK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 404 -61973 -153.40 -520.78 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -153.40 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.673

(partial model without unconserved sides chains): PDB file : Tito_1NGK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NGK-query.scw PDB file : Tito_Scwrl_1NGK.pdb: