Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYGIVLFPSKKLQDLANSYRKRYDPSYSLIPP-----HLTLRASFECAEEKADQLVSHLRNIAKESHPLVLKMTKYSSFAPVNN--VIYIKAEPTEELKTLNEKLYTGVLAG---------EQEYNFVPHVTVGQNLSDDEHSDVLGQLKMQEVSHEEIVDRFHLLYQL---ENGSWTVYETFLLGRGE 1IUH Chain:A ((1-183)) MRLFYAVFLPEEVRAALVEAQTKVRPFRGWKPVPPHQLHLTLLFLGERPEEELPDYLALGHRLARLEAPFRARLRGTGYFPNEGTPRVWFAKAEA-EGFLRLAEGLRAGVEELLGEEAVRIPGWDKPFKPHITLARRKAP------APRVPPVLFGLEWPVEGFALVRSELKPKGPVYTVLEKFSLRGEH

General information: TITO was launched using: RESULT: Template: 1IUH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 743 -102947 -138.56 -627.72 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -138.56 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_1IUH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IUH-query.scw PDB file : Tito_Scwrl_1IUH.pdb: