Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLAGGINPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCGALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPAKHKKPFILDMATSKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDK-VV-SLSTFGGPKGYGLSIVVDVFSGLLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAVGFERVYVPGEIEQLHEERNKKNGISIARSVYEFLKSR 2X06 Chain:A ((1-331)) ---MILKPENEKKLIIDVLKKFGVPEEDAKITADVFVDADLKGFTSHGIGRFPQYITALKLGNINPKPDIKIVKESPATAVIDGDLGLGQVVGKKAMELAIKKAKNVGVGVVATRNANHFGIAGYYSELAMNQDMIGITITNTEPAMAPFGGKEKILGTNPIAIAFKGN-KYKFSLDMATASIARGKILEALRKKIKIPEGCAVDKDGKPTTDPAKALEGCILPFGGPKGYGLALAIEMLSA-IGGAEVGTKVKGTA-NPEERCTKGDLFIAINPEFFMGKEEFKRKVDELLDEIKNSEPAEGF-EILIPGEIEERNKMKR-KDGFEIDKNLYNQLKEI

General information: TITO was launched using: RESULT: Template: 2X06.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1813 -123965 -68.38 -376.79 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -68.38 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_2X06.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X06-query.scw PDB file : Tito_Scwrl_2X06.pdb: