Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MVKYQYEFP----LDKAGKAGAVKPYRGGKNDFV---------TPVSNLSGVAEILTNAALK-----------ATEAYSQL------------------GQDRLGAVLISKVKGWAYADREGTLFIEESDNNNVWTTTAAVNVAAGVLTA-TDWVYLSKR----YYRFRYVNGNLQQSEFVLYQSVGAGEMDVRVNEKTPLQIDFAENQTHDGRLKVE-ARKTFDFV----------------------FHENAESASEGAALPVDGAAHLLVEVYGT---AEMSEVKFWGKSVSGQKLPIRGVKTDDATTASSTL-----GKAEAWAFDIKGFKEIIMEI-----ISITGGTLSVKGTAVS------ 4IND Chain:A ((6-383)) LGQSFPANAKVKYYYKLSEKQDLDAFVNSIFVGSYKLKQISYLLYGNTKIVSAPVVPLGPNASIIIDDELQEGLYLIRIKVYNTNSFSVTVTPFFNNNNTMTYSIGANSEFEIYDIFTKEQGNIYYIQLPPGLAILEFSLERVFEKGNRINIPKIIHTSGNGYISFRLRKGTYAIKMPYSYNNTTSTTFTNFQFGTISTSATIPLVISSIPANGSGSGTFLVYLKITGDYEDVKFSVTYGGGLGVPFTFGLEVEEINELVENTNFVTQSVTLSGSQVTQSILNVQGSGSHLRLKYASVSGLTTAVTQCQLQATNLNRSTTYSTVWDFIAGGSSTPPSWDIREINSIQLVANGGSSTSSVTITLILVYEQIAGELSHH

General information: TITO was launched using: RESULT: Template: 4IND.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1148 -107500 -93.64 -385.30 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -93.64 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.65 QMean score : -0.053

(partial model without unconserved sides chains): PDB file : Tito_4IND.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IND-query.scw PDB file : Tito_Scwrl_4IND.pdb: