Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MIHKHETRELVAIEELVMSWIEAFKSLSYFGIFLALSIEFIPAEVVLPLAGYWVSKGDMTLAGVVLAGSLGGVAGPLTLYWIGRYGGRPFLERFGKYLFIKPEALDKSDNFFKKHGGFVAFSGRFLPGIRTLISIPC---GIAKMNVWVFSLYTFIAMLPITFVYVYLGVKLGENWKAVGSILDQYMLPIGIAILALFLLYLLMKKRKK-----RTHSEQLSVFLKNKR-------- 4OHC Chain:A ((10-230)) TGYDRQSISDTTAKILLEVQAVHFNAEKPFIFTSGWASPVYIDCRKLISY-------PRVRRALMEMAETTITRDIG--FEQIDAVAGGETAGIPFAAWIADRMMVPMQYVRKKP---KGFGRNAQIEGHLEEGSRVLLVEDLTTDSRSKIN-FVNALRTAGATVNHCFVLFHYNI-----FKESVSVLK----DIDVDLHALATWWDVLRVAKASGYFETKTLDEVEKFLHAPAEWSAAHGG

General information: TITO was launched using: RESULT: Template: 4OHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 984 -177731 -180.62 -893.12 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -180.62 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_4OHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OHC-query.scw PDB file : Tito_Scwrl_4OHC.pdb: