Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELLATYLL-TEPGADTEKKAEQIATGLTVGSWTDLPLVKQEQMQKHKGRVIKVEEREGTA-----------ASEKQAVITIAYPEINFSQDIPALLTTVFGKLS-LD---GKIKLIDLHFSEAFKRALPGPKFGVYGIRKLLGEFERPLLMSIFKGVIGRDLSDIKEQLRQQALGGVDLIKDDEIFFETGLAPFETRIAEGKQILKETYEQTGHKTLYAVNLTGRTADLKDKARRAAELGADALLFNVFAYGLDVMQGLAEDPEIPVPIMAHPAVSGAFTSSPFYGFSHALLLGKLNRYCGADFSLFP-SPYGSVALPRADALAIHEECVRE-DAFNQTFAVPSAGIHPGMVPLLMRDFGI-DHIINAGGGVHGHPNGAQGGGRAFRAIIDAVL-EAQPIDEKAEQCKDLKLALDKWGKAEAV 2QYG Chain:A ((19-429)) DNYIELDFDFECAGD--PHEAAAHLCSEQSTAQWRRVG-FDEDFRPRFAAKVLELSAEPRPSGFSVPVECAARGPVHACRVTIAHPHGNFGAKIPNLLSAVCGEGVFFSPGIPLIRLQDIRFPEPYLAAFDGPRFGIAGVRERLQAFDRPIFFGVIKPNIGLPPQPFAELGYQSWTGGLDIAKDDEMLADVDWCPLAERAALLGDACRRASAETGVPKIYLANITDEVDRLTELHDVAVANGAGALLINAMPVGLSAVRMLRKH--ATVPLIAHFPFIAAFSRLANYGIHS-RVMTRLQRLAGFDVVIMPGFGPRMMTP-EHEVLDCIRACLEPMGPIKPCLPVPGGSDSAATLENVYRKVGSADFGFVPGRGVFGHPMGPAAGATSIRQAWDA-IAAGIPVPDHAASHPELAAALRAF------

General information: TITO was launched using: RESULT: Template: 2QYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2229 -235383 -105.60 -602.00 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -105.60 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_2QYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QYG-query.scw PDB file : Tito_Scwrl_2QYG.pdb: