Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSKIY-----------------VLDTNVLLQDPNAIF--SFEENEVVIPAVVLEEVDSKKRYMDEVGRNARHVSK--------LIDALRQKGRLHEHVPLDTGGTLRIELNHRSFHQLQEIFIEKTNDNR-----ILAVAKNLSLEEETKENGRPV-ILVSKDVLVRVK-----ADAIGLLAEDFLNDRVVDNDEMYSGYKDLYISQQLFSSFYGKNQISVNDVKQHAFYPNQFALMKDELGGSSSAVGIADKTGTVLKR-------LVFDDEHIWGIRPK--------NVQQTMALELLLREDIPLVTL--IGKAGTGKTLLALAAGLLQTEDLGIYKKLVVARPIVPVGKDIGYLPGEKEEKLKP---WMQPIFD--NLEFLFNAKKPGELDAILAG-----------IGSIQVEALTYIRGRSIPDQFIIIDEAQNLTRHEVKTLLTRVGEGSKIVLMGDPEQID----------HPYLDSLNNGLAYVVERFKGQPISGSVKLLKGERSGL------AQLAADLL---------------------------- 1QUV Chain:A ((1-556)) SMSYTWTGALITPCAAEESKLPINALSNSLLRHHNMVYATTSRSAGLRQKKVTFDRLQVLDDHYRDVLKEMKAKASTVKAKLLSVEEACKLTPPHSAKSKFGYGAKDVRNLSSKAVNHIHSVWKDLLEDTVTPIDTTIMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSTLPQVVMGSSYGFQ---YSPGQRVEFLVNTWKSKKNPMGFSYDTRCFDSTVTENDIRVEESIYQCCDLAPEARQAIKSLTERLYIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKASAACRAAKLQDCTMLVNGDDLVVICESAGTQEDAASLRVFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIY--GACYSIEPLDLPQIIERLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRVWRHRARSVRARLLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASLSGWFVAGYS

General information: TITO was launched using: RESULT: Template: 1QUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2006 -166210 -82.86 -380.34 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -82.86 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_1QUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QUV-query.scw PDB file : Tito_Scwrl_1QUV.pdb: