Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNPSKAEIKEILQRSKRIAVVGLSDRPDRTSHMVSKAMQDAGYE-IIPVNPTI--DEALGVKAVSSLKEIDG---PIDIVNVFRRSEQLPGVAEEFLETDAPVFWAQQ-G---LVNEEAYQMLKEKGRTVIMDLCIKVAHAVTK 2YV2 Chain:A ((9-155)) -----------LVDSETRVLVQGIT---GREGSFHAKAMLEYGTKVVAGVTPGKGGSEVHGVPVYDSVKEALAEHPEINTSIVFVPAPFAPDAVYEAVDAGIRLVVVITEGIPVHDTMRFVNYARQKGATIIGPNCPGAITPG--

General information: TITO was launched using: RESULT: Template: 2YV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 609 -4747 -7.79 -39.89 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -7.79 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_2YV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YV2-query.scw PDB file : Tito_Scwrl_2YV2.pdb: