Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVIHNGKAGLLGLSVQDVPSTKPGYGEVKVKLKSAGLNHRDLFLMKNK-------------------SEQDPHMILGSDGAGIIEEIGEGVKNVTVQTEVVIFPTLNWDLTENVP-------PVPEILGGP-SDGTLAEYVIIPSQNAIKKPAYLSWEEAGVLPLSALTAYRALFT--KGQLKKGEHLLIPGIGSGVATYALFMAKAIGATVSVTSRSEEKRKKALKLGADYAFDSYS-NWDEQ---------------------LQGKKIDVVLDSIGPALFSEYFRHVKPNGRIVSFGASSGDNLSFPVRSLFFPQVNVLGTSMGSGEEFQAMLAFIDKHKLRPVIDRIYPLEKACEAYKRMQEGRQFGNIGIVME 4A0S Chain:A ((49-413)) -------------SLRLGEVPMPELAPDEVLVAVMASSINYNTVWSAMFEPIPTFHFLKQNARQGGWATRHDQPYHVLGSDCSGVVVRTGIGVRRWKPGDHVIVHPAHVD-EQEPATHGDGMLGTEQRAWGFETNFGGLAEYGVVRASQLLPKPAHLTWEEAAVSPLCAGTAYRMLVSDRGAQMKQGDIVLIWGASGGLGSYAIQFVKNGGGIPVAVVSSAQKEAAVRALGCDLVINRAELGITDDIADDPRRVVETGRKLAKLVVEKAGREPDIVFEHTGRVTFGLSVIVARRGGTVVTCGSSSGYLHTFDNRYLWMKLKKIVGSHGANHEEQQATNRLFESGAVVPAMSAVYPLAEAAEACRVVQTSRQVGKVAVLC-

General information: TITO was launched using: RESULT: Template: 4A0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1909 -12731 -6.67 -40.54 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -6.67 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_4A0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A0S-query.scw PDB file : Tito_Scwrl_4A0S.pdb: