Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTYVVDDSKYKCIYAGTEKAAAFNNEFENGTRVRVWFEGHHIKTFEKEQREVCGVGEWIVKYDAATELQKEVNRLEKTYFK---KKELLDTIRQADGVGREINEKKKSILNYSGC 2N3Z Chain:A ((1-99)) MGRLVVVVTS-------EQLKEEVRKKFPQ-VEVRLVTTEEDAKQVIKEIQKK-GVQKVVLVGVSEKLLQKIKQEANVQVYRVTSNDELEQVVKDVKGSGLEHHHHHH--------

General information: TITO was launched using: RESULT: Template: 2N3Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 414 -21532 -52.01 -224.29 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -52.01 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_2N3Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N3Z-query.scw PDB file : Tito_Scwrl_2N3Z.pdb: