Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKTMKDIQAEVDRYIGQFKEGY-FSPLAMMARLTEELGELAREVNHRYGEKPKK---ATEDDKSMEEEIGDVLFVLVCLANSLDISLEEAHDRVMHKFNTRDKDRWTRKEEGK 2A3Q Chain:A ((26-134)) SPEPTLEDIRRLHAEFAAERDWEQFHQPRNLLLALVGEVGELAELFQWKSDTEPGPQAWPPKERAALQEELSDVLIYLVALAARCHVDLPQAVISKMDTNRQRYPVHLS------

General information: TITO was launched using: RESULT: Template: 2A3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 235 -20122 -85.63 -191.64 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -85.63 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_2A3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A3Q-query.scw PDB file : Tito_Scwrl_2A3Q.pdb: