Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKYTIVDKDTCIACGACGAAAPDIYDYDDEGIAFVTLDENKGVVEVPEVLEEDMIDAFEGCPTDSIKVADEPFEGDPLKFE 1WTF Chain:A ((1-81)) -PKYTIVDKETCIACGACGAAAPDIYDYDEDGIAYVTLDDNQGIVEVPDILIDDMMDAFEGCPTDSIKVADEPFDGDPNKFE

General information: TITO was launched using: RESULT: Template: 1WTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 394 -43933 -111.51 -542.38 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.96 3D Compatibility (PKB) : -111.51 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.688

(partial model without unconserved sides chains): PDB file : Tito_1WTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WTF-query.scw PDB file : Tito_Scwrl_1WTF.pdb: