Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDFLSKTPEPPYYAVIFSSVKSENDTGYGETAERMVSLAADQPGFLGVESVREADGRGITV-SYWDSMDAINHWRHHTEHQAAKEKGRSVWYESYAVRVAKVDRQRLFQENTND 1Y0H Chain:A ((1-101)) -------GMTSPVAVIARFMPRPDARSALRALLDAMITPTRAEDGCRSYDLYESADGGELVLFERYRSRIALDEHRGSPHYLNYRAQVGELLTRPVAVTVLAPLDEAS-------

General information: TITO was launched using: RESULT: Template: 1Y0H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 374 -23279 -62.24 -232.79 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -62.24 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_1Y0H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y0H-query.scw PDB file : Tito_Scwrl_1Y0H.pdb: