Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTATIIDGKETAREKREQLAKEVEELKKQ-GVTPGLAVILIGDDPASHSYVRGKKKAAETMGMNFKLDQFDSSLTEAELLSIIDQYNQDPEFHGILVQLPLPDH-ISEKAVIERISPDKDVDGFHPLNVGKMLLGEDT-FLPCTPHGIVELLKKTNIDLSGKEVVVVGRSNIVGKPVGQLLLNENATVTYCHSRTENITEHTKKADILVVAVGRANFISADQIKEGAVVIDVGVNRLENGKLCGDVEFEGAKEKASFITPVPGGVGPMTITMLAHNTVKSAKRTLS---- 1B0A Chain:A ((2-288)) -AAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSNPASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLPA-GIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAP-RLRPCTPRGIVTLLERYNIDTFGLNAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPGDWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTLQACVEYHDPQDE

General information: TITO was launched using: RESULT: Template: 1B0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1518 3725 2.45 13.30 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 2.45 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_1B0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B0A-query.scw PDB file : Tito_Scwrl_1B0A.pdb: