Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIAEVLPESMKNALSEIPEQQWLEIEEVRIRINRPVELIRRGQPVY-LSYAGTAEDAHLILSRLSNYSMYTLE--EELKKGYVTIRGGHRVGLAGRVITENG-GVKGLRDIASFNIRIARQK-LGI-A----------------------EPLLPYLYQN--SWLNTLIIGPPQTGKTTLLRDLARLSSTGKKNMLPVKTGIVDERSEIAGCLR-GIPQHQFGQRIDVLDACPKAEGLMMMIRSMSPEVMIVDEIGRMEDTDALLEALHAGVS-VIVSAHGWSISDLMKRPSLKRLWE---------------ERAFDRYLELSRAK---GPGTVSQIYDKDGNVLSRTTGVKTC 2GZA Chain:A ((33-346)) ---------------------LDDPQITEVCVNRPGEVFCERASAWEYYAVPNLDYEHLISLGTATARFVDQDISDSRPVLSAIL--PMGERIQIVRPPACEHGTISVTIRKPSFTRRTLEDYAQQGFFKHVRPMSKSLTPFEQELLALKEAGDYMSFLRRAVQLERVIVVAGETGSGKTTLMKALMQEIPFDQ------RLITIEDVPELFLPDHPNHVHLFYP--------VTAATLLRSCLR-MKPTRILLAELRGGE-AYDFINVAASGHGGSITSCHAGSCELTFERLALMVLQNRQGRQLPYEIIRRLLYLVVDVVVHVHNGVHDGTGRHISEVWYD--------------

General information: TITO was launched using: RESULT: Template: 2GZA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1125 -9858 -8.76 -38.81 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -8.76 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_2GZA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GZA-query.scw PDB file : Tito_Scwrl_2GZA.pdb: