Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MMIKQCVICLSLLVFGTTAAHAEETPLVTARHMSKWEEIAVKEAKKRYPLAQVL-----FKQKVWDRKR------------KDEAVKQYHLTLREGSKEF---------------GVFVTISFDP--YSQKVNKIAILEEYQ 1HD2 Chain:A ((1-161)) APIKVGDAIPAVEVFEGEPGNKVNLAELFKGKKGVLFGVPGAF---TPGCSKTHLPGFVEQAEALKAKGVQVVACLSVNDAFVTGEWGRAHKAEGKVRLLADPTGAFGKETDLLLDDSLVSIFGNRRLKRFSMVVQDGIVKALNVEPDGTGLTCSLAPNIISQL

General information: TITO was launched using: RESULT: Template: 1HD2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 443 -3605 -8.14 -34.33 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -8.14 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_1HD2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HD2-query.scw PDB file : Tito_Scwrl_1HD2.pdb: