Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNR----------TLEKAKRLTEATPSFHNKEVLSIKEAEE-RLEQYDVIIHT--TSVGMYPNVDDVPL-SLQRAASSAVVCDIVYNPIQTALLKEASQKGLKTLDGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC 3TNL Chain:D ((38-315)) --ELIGLIATPIRHSLSPTMHNEAFAKLGLDYVYLAFEVGDKELKDVVQGFRAMNLRGWNVSMPNKTNIHKYLDKLSPAAELVGAVNTVVNDDGVLTGHITDGTGYMRALKEAGHDIIGK-KMTICGAGGAATAICIQAALDGVKEISIFNRKDDFYANAEKTVEKINSKTDC-----KAQLFDIEDHEQLRKEIAESVIFTNATGVGMKPFEGETLLPSADMLRPELIVSDVVYKPTKTRLLEIAEEQGCQTLNGLGMMLWQGAKAFEIWTHKEMPVDYIKEILF--------

General information: TITO was launched using: RESULT: Template: 3TNL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1507 -71654 -47.55 -271.41 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -47.55 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3TNL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TNL-query.scw PDB file : Tito_Scwrl_3TNL.pdb: