Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLKMLSMLTVMIASLFIFSSQALAVQYFTVSTSSGAPVNMRSGPGTNYPIMLSIPSGSRVPYYCYAYGTTVTGKYGTSNIWDQIQW-KDSRGVVNIGYVSDTYVYTGSDGPVGYKCN 4KRT Chain:A ((295-351)) ------------------------------------STFLNVREEGSLNSRIVDKINSGDIFRIDWVD---------SDFIGWYRITTKNGKVGFVNAEFVK-----------------

General information: TITO was launched using: RESULT: Template: 4KRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 213 -4255 -19.98 -75.98 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -19.98 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_4KRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KRT-query.scw PDB file : Tito_Scwrl_4KRT.pdb: