Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEQPIGVIDSGVGGLTVAKEIMRQLPKENIIYVGDTKRCPYGPRPEEEVLQYTWELTNYLLENHHIKMLVIACNTATAIALDDIQRSVGIPVVGVIQPGARAAIKV--TDNQHIGVIGTENTIKSNAYEEALLALNPDLKVENLACPLLVPFVESGKFLDKTADEIVKTSLYPLKD-TSIDSLILGCTHYPILKEAIQRYMGEHVNIISSGDETAREVSTILSYKGLLNQSPIAPDHQFLTTGARDQFAKIADDWFGHEVGHVECISLQEPIKR 3UHO Chain:B ((26-229)) ----KIGVFDSGVGGLSVLKSLYEARLFDEIIYYGDTARVPYGVKDKDTIIKFCLEALDF-FEQFQIDMLIIACNTASAYALDALRAKAHFPVYGVIDAGVEATIKALHDKNKEILVIATKATIKSEEYQKRLLS-QGYTNINALATGLFVPMVEEGIF---EGDFLQSAMEYYFKNITTPDALILACTHFPLLGRSLSKYFGDKTKLIHSGD--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1074 -91378 -85.08 -454.62 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -85.08 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_3UHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UHO-query.scw PDB file : Tito_Scwrl_3UHO.pdb: