Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRADFIQFGAMIHGVGGTTDGWRHPDVDPSASTNIEFYMKKAQTAEKGLFSFIFIADGLFISEKSIPHFLNRFEPITILSALASVTKNIGLVGTF-STSFTEPFTISRQLMSLDHISGGRAGWNLVTSPQEGAARNHSKSNLPEHTERYEIAQEHLDVVRGLWNSWEHDAFIHNKKTGQFFDQAKLHRLNHKGKYFQVEGPLNIGRSKQ-GEPVVFQAGSSETGRQFAAKNADAIFTHSN-SLEETKAFYADVKSRAADEGRDPSSVRIFPGISPIVADTEEEAEKKYREFAELIPIENAVTYLARFFDDYDLSVYPLDEPFPDIGDVGKNAFQSTTDRIKREAKARNLTLREVAQEMAFPRTLFIGTPERVASLIETWFNAEAADGFIVGSD-I------PGTLDAFVEKVIPILQERGLYRQDYRGGTLRENLGLGIPQHQSVLHSSHH 1LUC Chain:B ((1-320)) ------MKFGLFFLN--FMNS----K---RSSDQVIEEMLDTAHYVDQLKFDTLAVYENHFSNNG------VVGAPLTVAGFLLGMTKNAKVASLNHVITTHHPVRVAEEACLLDQMSEGRFAFGFSDCEKSADMRFFNR----PTDSQFQLFSECHKIINDAFTT---------------------GYCHPNNDFYSFPKISVNPHAFTEGGPAQFVNATSKEVVEWAAKLGLPLVFRWDDSNAQRKEYAGLYHEVAQAHGVDVSQVRHKLTLLVNQNVDGEAARAEARVYLEEFVR---------ESY------SNT-----------------------DF----EQKMGEL------LSENAIGTYEESTQAARVAIECCGAADLLMSFESMEDKAQQRAVIDVVNANIV--------------------------------------

General information: TITO was launched using: RESULT: Template: 1LUC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1651 -36355 -22.02 -117.27 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -22.02 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_1LUC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LUC-query.scw PDB file : Tito_Scwrl_1LUC.pdb: