Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------MAKKDEHLRKPEWLKIKL----------NTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTAT-----FMILGSVCTRACRFCAVK--TGLPTELDLQEPERVADSVALMNLKHAVITAVARDDQKDG----GAGIFAETVRAIRRKSPFTTIEVLPSDMGGNYDNLKTLMDTRPDI---LNHNIETV-RRLTPRVRARATYDRSLEFLRRAKEMQPDIPTKSSIMIGL-GETKEEIIEVMDDLLANNVDIMAIGQYLQ---------PTKKHLKVQKYYHPDEFAELKEIAMQKG-----------FSHCEAGPLV-RSSYHA---DEQVNEASKKRQAQA--------------------- 4JC0 Chain:A ((2-433)) RVGIKVLGCPKNEADCEVLAGVLREGGHEIVFDVKDADVVVLDTCAFIEDAKRESIDEIFSFVDAKDQYGYKLVVKGCLVQRYYEELKKEIPEVDQWIGVADPEEIANAIETDLVPDRYRKRIDLEERPYAYVKISDGCDRGCTFCSIPSFKGSLRSRSIEDITREVEDLLKEGKK--EIILVAQDTTSYGIDLYRKQALPDLLRRLNSLNGEFWIRVMYLHPDHLTEEIISAMLELDKVVKYFDVPVQHGSDKILKLMGRTKSSEELKKMLSSIRERFPDAVLRTSIIVGFPGETEEDFEELKQFVEEIQFDKLGAFVYSDEEGTVAFNLKEKVDPEMAKRRQEELLLLQAEISNSRLDRFVGKKLKFLVEGKEGKFLVGRTWTEAPEVDGVVFVRGKGKIGDFLEVVIKEHDEYDMWGSVILEH

General information: TITO was launched using: RESULT: Template: 4JC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1465 104146 71.09 351.84 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 71.09 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_4JC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JC0-query.scw PDB file : Tito_Scwrl_4JC0.pdb: