Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTASKFSVMFFMLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQNLLPQSEDSQASGKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKI--MIPLRIGRASVYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVGFLSADETQTLSWVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 4NDZ Chain:A ((371-656)) --DEEDDVVIIYNRVPKTA---------STSFTNIAYDLCAKNRYHVLHINTTKNNPVMS----------LQDQVRFVKNVTSWKEMKPGFYHGHVSYLDFAKF---GVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAAGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWALEQAKYNLIN-EYFLVGV----TEELEDFIMLLEAALPRFFRGATELYRTGKKSH-------LRKTTEKKLP----------------------TKETIAKLQQSEIWKMENEFYEFALEQFQFVRAHAVREKDGELYILAQNFFYEKIYPK--------------------------------

General information: TITO was launched using: RESULT: Template: 4NDZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1315 12007 9.13 42.28 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 9.13 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_4NDZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NDZ-query.scw PDB file : Tito_Scwrl_4NDZ.pdb: