Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MHDYIKERTIKIGKYIVETK------KTVRVIAKEFGVSKSTVHKDLT--ERLPEINPDLANEVK---EILDYHKSIRHLRGG----EATKLKYKKDEILEGEPVQQS-- 2AO9 Chain:A ((13-132)) MMAKLDELKQKLTAKQIQAAYLLVENELMEEEKRTQDEMANELGINRTTLWEWRTKNQDFIAFKSEVADSFLAEKREQVYSKLMQLILGPQPSVKAMQLYMQRFGLLTDKKVIEGDL

General information: TITO was launched using: RESULT: Template: 2AO9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 238 19773 83.08 212.61 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 83.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_2AO9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AO9-query.scw PDB file : Tito_Scwrl_2AO9.pdb: