Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTIFKQKNTHPFSNAANRLDRLPISRVHFQVLTALGIVYFFDLADLFTLSNVAPALIE------HWGIPLSTIANVTAASFLGMFLGASLGGRLSDRI-GRKKALNLFVFVFSIASLCNAAAW--DIPSLMTFRFLTGFGVAAAMVITNSYLAEFFPS--SVRGKYISFCAMIGLIGVPITNIVSAFVIPLGSWGWRLVFVWGAVGLIY-FFFIH--RLEESPRWHENRGEYAKADAILTRIEEQVEKEKGPL--------------P---AASQPKVSE---------------TVKQNAGYAGLLKGRNLKI-TIVLSAVWIFETFGFYGFASWVPSLLKSN-GVT----MENTLWYNVLHSVGAPLGALLGSMISERFQRKWI------LAASAFLTAIAGLLYGMT-----------FIPIMIIVFGFIVNITERVFTSNLYAYTSEPYPTEYRSSGSGLAYGLGRFSNIFGSLLVGFIAVQ--LGYISVFLFIGGCWLACSLLLIFFGPNTNAKQI 4W6V Chain:A ((27-490)) ---------------------------------------EMWERFSFYGIRPLLILFMAATVFDGGMGLPREQASAIVGIFAGSMYLAALPGGLLADNWLGQQRAVWYGSILIALGHLSIALSAFFGNDLFFIGLVFIVLGTGLFKTCISVMVGTLYKPGDARRDGGFSLFYMGINMGSFIAPLLSGWLLR--THGWHWGFGIGGIGMLVALLIFRGFAIPAMKRYDAEVGLDSSWNK----PT------GRWVTAIMAVVVVIIALISQGVIPINPVMIASLLVYVIAASVTLYFIYLFAFA-KMSRKDRARLLVCFILLVSAAFFWSAFEQKPTSFNLFANDYTDRMVMGFEIPTVWFQSINALFIILLAPVFSWA---------PSSITKFVIGILCAAAGFAVMMYAAQHVLSSGGAGVSPLWLVMSILLLTLGELCLSPIGLATMTLLAPDRMRGQVMGLWFCASSLGNLAAGLIGGHVKADQLDMLPTLFARCSIALVICAAV-------------

General information: TITO was launched using: RESULT: Template: 4W6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1496 -209348 -139.94 -555.30 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -139.94 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_4W6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W6V-query.scw PDB file : Tito_Scwrl_4W6V.pdb: