Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRTEKEKMAAGELYNSE-DQQLLLERKHARQLIRQYNET-PEDDAVRTKLLKELLGSVGDQVTILPTFRCDYGYHIHIGDHTFVNFDCVILDVCEVRIGCHCLIAPGVHIYTAGHPLDPIERKSGKEFGKPVTIGDQVWIGGRAVINPGVTIGDNAVIASGSVVTKDVPANTVVGGNPARILKQL 4EGG Chain:F ((2-184)) ---TEKEKMLAEKWYDANFDQTLINERLRAKVICFALNHTNPVATMMRKVLIDALFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKI

General information: TITO was launched using: RESULT: Template: 4EGG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 941 -15642 -16.62 -86.42 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain F : 0.84 3D Compatibility (PKB) : -16.62 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.651

(partial model without unconserved sides chains): PDB file : Tito_4EGG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGG-query.scw PDB file : Tito_Scwrl_4EGG.pdb: