Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRRLAIVQRWRGQDEIPTDWGRCVLTIGVFDGVHRGHAELIAHAVKAGRARGVPAVLMTFDPHPMEVVYPGSHPAQLTTLTRRAELVQDLGIEVFLVMPFTTDFMK---LTPDRFIHELLVEHLHVVEVVVGENFTFGKKAAGNVDTLRRAGERFGFAVESMSLVSEHHSNETVTFSSTYIRSCVDAGDMVAAMEALGRPHRVEGVVVRGEGRGAELGFPTANVAPPMYSAIPAD-GVYAAWFTVLGHGPVTGTVVPGERYQAAVSVGTNPTFSGRTRTVEAFVLDTTADLYGQHVALDFVGRIRGQKKFESVRQLVAAMGADTERARDLLSTG 1S4M Chain:B ((1-282)) -----------------------MVVSIGVFDGVHIGHQKVLRTMKEIAFFRKDDSLIYTI-SYPPEYFLP-DFPGLLMTVESRVEMLSRYARTVVL------DFFRIKDLTPEGFVERYLS---GVSAVVVGRDFRFGKNASGNASFLRKKGVEV-YEIEDVVVQGKR-------VSSSLIRNLVQEGRVEEIPAYLGRYFEIEGIVHK---------FPTANIDRGNEKLVDLKRGVY----LVRVHLP------DGKKKFGVMNVGF-------NVKYEVYILDFEGDLYGQRLKLEVLKFMRDEKKFDSIEELKAAIDQDVKSARNMID--

General information: TITO was launched using: RESULT: Template: 1S4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -191532 for 2042 contacts (-93.8/contact) + 2D Compatibility (PS) -27678 + (NN) -5172 + (LL) 3304 1D Compatibility (HY) -24800 + (ID) 4600 Total energy: -250478.0 ( -122.66 by residue) QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1S4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S4M-query.scw PDB file : Tito_Scwrl_1S4M.pdb: