Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFA--LRSMVPQEIAKDDFQSYPLWRAFNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLRTRAEGKNSWAAKQIDRCVEGLSSP----IFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSLRRFADRLMVKLNHIRIK 1KNQ Chain:A ((9-173)) HIYVLMGVSGSGKSAVASEVAHQLHA-AFLDGDFLHPRRNIEKMASGEP--LNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSAL-KKHY----RDLLREGNPNLSFIYLKGDFDVIESRLKARKGHFF--KTQMLVTQFETLQEPGADETDVLVVDIDQ-PLEGVVASTIEVIKK---------------------------

General information: TITO was launched using: RESULT: Template: 1KNQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 738 -103916 -140.81 -653.56 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -140.81 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_1KNQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KNQ-query.scw PDB file : Tito_Scwrl_1KNQ.pdb: